DNA

Part:BBa_K156008:Design

Designed by: George McArthur IV   Group: iGEM08_Virginia   (2008-10-27)

BioBrick placeholder 8 (Prefix - MfeI (NotI) SbfI - Suffix)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 45
    Illegal PstI site found at 38
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 46
    Illegal PstI site found at 38
    Illegal PstI site found at 60
    Illegal NotI site found at 7
    Illegal NotI site found at 29
    Illegal NotI site found at 53
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 46
    Illegal PstI site found at 38
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 46
    Illegal PstI site found at 38
    Illegal PstI site found at 60
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sites are compatible with standard BBa restriction sites and will form scars when ligated.


Source

The DNA sequence is simply linked restriction enzyme sites that are compatible with standard BBa restriction sites. These compatible sites were compiled and listed in "Engineering BioBrick vectors from BioBrick parts". http://www.jbioleng.org/content/2/1/5

References

Shetty RP, Endy D, Knight TF Jr. (2008). Engineering BioBrick vectors from BioBrick parts. J Biol Eng 2: 5.